Optimization of Quantitative PCR Assays for Rapid and Sensitive Detection of Gardnerella Vaginalis and Associated Anaerobes in Bacterial Vaginosis
DOI:
https://doi.org/10.29070/fwqwd962Keywords:
Bacterial vaginosis (BV), Gardnerella vaginalis, Anaerobes, Atopobium vaginae, BVAB2, Megasphaera, Quantitative PCR (qPCR)Abstract
The disadvantages of conventional procedures for diagnosing bacterial vaginosis (BV), such as Nugent scoring and Amsel's criteria, include a lack of accessibility, the need for specialist expertise, and a high degree of subjectivity. The most prevalent gynaecological infection in the world today is still bacterial vaginosis (BV). The purpose of this work is to enhance quantitative polymerase chain reaction (qPCR) tests in order to identify the presence of Gardnerella vaginalis and other anaerobes that are linked with bacterial vaginosis (BV), such as Atopobium vaginae, BVAB2 (a member of the Clostridiales family that has not been cultivated), and Megasphaera phylotypes 1 and 2. The objective is to surmount these obstacles. The test was fine-tuned, primer-probe efficiency was increased, and quantitative cutoffs were established for more accurate identification with the aid of an analysis of vaginal samples. The improved quantitative polymerase chain reaction (qPCR) assays performed better than traditional diagnostic approaches and shown good sensitivity and specificity in diagnosing symptomatic bacterial vaginosis (BV). Two significant advantages of the streamlined method are the reduction of dependency on subjective rating systems and the acceleration of turnaround times. According to the findings, optimised molecular tests have the potential to be a quick, accurate, and clinically useful substitute for the present techniques of bacterial vaginosis diagnosis.
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